Coding

Part:BBa_K4164994:Design

Designed by: Bo Cheng   Group: iGEM22_NAU-CHINA   (2022-10-03)


FXR-3*GS Linker-ddRFPA1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1468
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1057


Design Notes

The stop codon of the BBa_K4164002 was deleted, and a flexible linker (3*GGGGS) was added between FXR and ddRFPA1.


Source

This sequence is composed of BBa_K416002and BBa_K4164004.

References